Coding

Part:BBa_K2933025:Design

Designed by: Wenhui Gong   Group: iGEM19_TJUSLS_China   (2019-09-05)


subclass B1 beta-lactamase II [Erythrobacter litoralis HTCC2594], codon optimized in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 484
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized in E. coli BL21(DE3).

Source

NCBI

References